Scientific Researches On:
Grifola Frondosa (Maitake Mushroom)
USA National Center for Biotechnology Information
41:
J Pharmacobiodyn.
1986 Oct;9(10):861-4.
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Antitumor activity of a
beta-1,3-glucan obtained from
liquid cultured mycelium of
Grifola frondosa.
Ohno N,
Adachi Y,
Suzuki I,
Oikawa S,
Sato K,
Ohsawa M,
Yadomae T.
The antitumor activity of a
branched beta-1,3-glucan "grifolan
LE" purified from liquid
cultures of Grifola frondosa (Ohno
et al. Chem. Pharm. Bull., 34,
1709-1715 (1986] was examined
on an allogeneic murine tumor
system. By intraperitoneal (i.p.)
administration (100-200
micrograms/mouse/d X 5) at
days 1 to 9 from the tumor
transplantation, grifolan LE
showed marked inhibitory
activity on the growth of
solid form sarcoma 180 in ICR
mice. Significant activity was
also observed in intravenous (i.v.)
or intratumoral (i.t.)
administrations. However, the
oral (p.o.) administration of
grifolan LE was not effective.
I.p. administration of
grifolan LE at a dose of 100
micrograms/mouse/d X 5 before
the tumor transplantation
showed significant inhibition
of tumor growth. I.p.
administration of grifolan LE
at day +11 to +19 was also
effective. Grifolan LE was not
effective on the ascites form
of sarcoma 180. The
pretreatment of sarcoma 180
cell with grifolan LE in vitro
did not affect tumor growth.
The mice cured from the solid
form of sarcoma 180 by
administration of grifolan LE
had the ability to reject the
same tumor cell. From these
results, it is suggested that
the antitumor activity of
grifolan LE occurred by
modification of biological
responses.
PMID: 3820062 [PubMed -
indexed for MEDLINE]
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Structure-function
Relationship of antitumor
beta-1,3-glucan obtained from
matted mycelium of cultured
Grifola frondosa.
Iino K,
Ohno N,
Suzuki I,
Sato K,
Oikawa S,
Yadomae T.
PMID: 3830423 [PubMed -
indexed for MEDLINE]
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Structural characterization
and antitumor activity of the
extracts from matted mycelium
of cultured Grifola frondosa.
Ohno N,
Iino K,
Takeyama T,
Suzuki I,
Sato K,
Oikawa S,
Miyazaki T,
Yadomae T.
PMID: 4085071 [PubMed -
indexed for MEDLINE]
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Neutral and acidic
antitumor polysaccharides
extracted from cultured fruit
bodies of Grifola frondosa.
Ohno N,
Iino K,
Suzuki I,
Oikawa S,
Sato K,
Miyazaki T,
Yadomae T.
Publication Types:
PMID: 4040819 [PubMed -
indexed for MEDLINE]
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Effect of a polysaccharide
fraction from Grifola frondosa
on immune response in mice.
Suzuki I,
Itani T,
Ohno N,
Oikawa S,
Sato K,
Miyazaki T,
Yadomae T.
The biological and
immunomodulating activities of
polysaccharide fraction
(GF-1), an antitumor
poysaccharide fraction from
cultured fruiting bodies of
Grifola frondosa, was examined
in mice. GF-1 showed no
cytocidal effect on culturing
tumor cells. However, GF-1
induced resistance against
Sarcoma 180 in ICR mice which
had completely regressed from
the tumor by the effect of
GF-1. The administration of
GF-1 into mice increased the
weights or cell numbers of
spleen, and peritoneal cavity.
GF-1 enhanced the antigen
specific antibody response and
carbon clearance activity,
whereas GF-1 did not show
polyclonal B cell activation
and mitogenic activities, and
the effect on delayed type
hypersensitivity.
PMID: 3891963 [PubMed -
indexed for MEDLINE]
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-
Antitumor activity of a
polysaccharide fraction
extracted from cultured
fruiting bodies of Grifola
frondosa.
Suzuki I,
Itani T,
Ohno N,
Oikawa S,
Sato K,
Miyazaki T,
Yadomae T.
Antitumor activity of a
polysaccharide fraction (GF-1)
extracted from cultured
fruiting bodies of a fungus,
Grifola frondosa, was examined
on allogeneic and syngeneic
tumors in mice. GF-1 had a
marked inhibitory activity
against the growth of
subcutaneously (s.c.)
inoculated Sarcoma 180 by the
intraperitoneally (i.p.)
injection at 0.5-5.0 mg/mouse
for 10 successive days. A
significant antitumor activity
was also observed when GF-1 at
4.0 mg/mouse was i.p. injected
successively on days +1-+5,
+7-+11, +14-+18 or +21-+25 if
the tumor cells were
inoculated s.c. on day 0.
Similar results were obtained
by a single i.p. injection of
GF-1 at 2.0 mg/mouse on day
+1, +7, +14 or +21. When GF-1
was injected i.p.,
intravenously (i.v.) or
intratumorally (i.t.), all of
them showed an equivalent
level of higher inhibitory
activity (inhibition ratio;
over 90%). However, the oral (p.o.)
administration was not
effective. The pretreatment of
mice with GF-1 at 2.0 or 4.0
mg/mouse for 5 times before
tumor inoculation did not show
a significant antitumor
activity. In addition, GF-1
administered i.p. at varying
times before and/or after the
tumor inoculation showed no
antitumor activity against
ascites form of Sarcoma 180.
In the syngeneic systems, GF-1
exhibited an antitumor
activity against solid form of
Meth A fibrosarcoma in BALB/c
mice and MM46 carcinoma in
C3H/He mice.
PMID: 6491867 [PubMed -
indexed for MEDLINE]
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[Studies on antitumor
activities of
Basidiomycetes-antitumor
activity of polysaccharides
and sex factors]
[Article in Japanese]
Ito H,
Naruse S,
Sugiura M.
We have already reported
antitumor activities of fungal
and bacterial polysaccharides
on mice. In the present
experiment, the influence of
the sex on antitumor effects
on such material from Grifola
umbellata, Coriolus versicolor
Fries or Sargassum thumbergii
and the immunity of mice
against tumor were
investigated. The growth
velocities of Sarcoma 180,
Ehrlich solid carcinoma,
Pulmonary tumor 7423 and
MF-sarcoma bearing mice both
without treatment and those
treated with polysaccharides
were more rapid in males than
in females. The regression
rates in mice with the above
tumors were higher in females
than in males. However, a few
DS Mie mice with Sarcoma 180
and A/Jax Mie mice with
Ehrlich solid carcinoma
regressed spontaneously. The
growth velocity of Shionogi
carcinoma 42 was not
influenced by the sex. On
other hand, both males and
females which had experienced
a regression of ascites tumor
after the administration of
polysaccharides rejected the
re-implanted Ehrlich ascites
carcinoma, Sarcoma 180, NF-sarcomma
and Shionogi carcinoma 42.
These results suggest that a
strong ehancement of immune
response occurs in the tumor
implanted in the host animal
by the administration of
polysacchrides. The
combination of X-ray
irradiation Ehrlich ascites
cells and polysacchrides
strengthens the antitumor
effect of NF-sarcoma and
Shionogi carcinoma 42.
Peritoneal exudate cells and
lymphocytes were compared
between the male and female
mice after being treated with
ATSO and P.GU-1. Such cells
were present to a much greater
extent in females.
Publication Types:
PMID: 986353 [PubMed - indexed
for MEDLINE]
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